The human beta-interferon gene enhancer is under negative control.

S Goodbourn; H Burstein; T Maniatis

doi:10.1016/0092-8674(86)90292-8

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The human beta-interferon gene enhancer is under negative control.

S Goodbourn·H Burstein·T Maniatis

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Abstract

The human beta-interferon gene is regulated by an inducible enhancer element. Analysis of the effect of deletions within this element on beta-interferon transcription indicates that this enhancer is under negative control. Deletion of sequences from the 3' end of the enhancer leads to a dramatic increase in the basal level of beta-interferon mRNA and a decrease in the induction ratio. The remaining 5' region of the enhancer can act as a strong constitutive transcription element, and it shares considerable homology with sequences known to be required for the activity of constitutive viral enhancers. We conclude that the beta-interferon enhancer consists of a constitutive transcription element and a negative regulatory sequence that prevents enhancer activity prior to induction. Thus, derepression of a constitutive transcription element appears to play a key role in the control of human beta-interferon gene expression.

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Animals Base Sequence Cell Line Chromosome Deletion Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Genes, Viral Humans Interferon Type I Mice Promoter Regions, Genetic RNA, Messenger Sequence Homology, Nucleic Acid Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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S Goodbourn, H Burstein, T Maniatis. 1986-05-23. The human beta-interferon gene enhancer is under negative control.. https://doi.org/10.1016/0092-8674(86)90292-8

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