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New, sensitive thin-layer chromatographic-high-performance liquid chromatographic method for detection of trichothecene mycotoxins.

B Yagen·A Sintov·M Bialer
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Abstract

Diphenylindenone sulphonyl (Dis) esters of trichothecene mycotoxins when sprayed with sodium methoxide showed fluorescent spots on a thin layer of silica gel when viewed under long-wavelength UV light. The detection limit for trichothecene esters in thin-layer chromatography (TLC) was 20-25 ng per spot for T-2 toxin, HT-2 toxin, diacetoxyscirpenol, T-2 triol, T-2 tetraol and iso-HT-2 toxin. A quantitative high-performance liquid chromatographic (HPLC) analysis of Dis-trichothecene esters was also developed using UV detection at 278 nm. The detection limit for the above esters varied between 30 and 50 ng per injection. This sensitive TLC-HPLC method is very useful for in vivo pharmacokinetic analyses of trichothecenes.

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Chromatography, High Pressure LiquidChromatography, Thin LayerMicrochemistryMycotoxinsSesquiterpenesSolventsStructure-Activity RelationshipTrichothecenesJournal ArticleResearch Support, Non-U.S. Gov't

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B Yagen, A Sintov, M Bialer. 1986-03-28. New, sensitive thin-layer chromatographic-high-performance liquid chromatographic method for detection of trichothecene mycotoxins.. https://doi.org/10.1016/s0021-9673(00)91479-1

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