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A comparative study of buffer systems as cryoprotectants for human spermatozoa.

G S Prins·L Weidel
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Abstract

Several cryoprotective media currently in use for human sperm cryopreservation have been directly compared with each other by use of a static vapor freezing technique. The TEST-citrate-yolk buffers with glycerol resulted in the highest motility and longevity after thawing; thus, their use is recommended.

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AnimalsBuffersCitratesCitric AcidCryoprotective AgentsEgg YolkFreezingGlycerolHEPESHumansHydroxidesIsotonic SolutionsMaleMilkPotassiumPotassium CompoundsSemen PreservationSperm MotilityTromethamineComparative StudyJournal Article

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G S Prins, L Weidel. 1986. A comparative study of buffer systems as cryoprotectants for human spermatozoa.. https://doi.org/10.1016/s0015-0282(16)49476-8

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