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Endotoxin-inactivating activity in normal and pathological human blood samples.

T Obayashi·H Tamura·S Tanaka·M Ohki·S Takahashi·T Kawai
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Abstract

The endotoxin-specific chromogenic test revealed that plasma endotoxin-inactivating activity was markedly diminished by endotoxemia, but not by fungemia or by dialysis with cellulose membranes, suggesting that fungal polysaccharides and other nonendotoxic, Limulus-reactive materials do not consume endotoxin-inactivating factors in the blood. There was a close negative correlation between plasma endotoxin concentration and endotoxin-inactivating activity. The specificity of the test was improved by fractionating amebocyte lysate and using only the factors that constitute the endotoxin-sensitive coagulation pathway of the horseshoe crab. This test was able to differentiate endotoxemia from fungemia and from contamination with other nonendotoxic, Limulus-reactive materials.

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EndotoxinsHumansLimulus TestMycosesRenal DialysisJournal Article

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BibTeXRIS

T Obayashi, H Tamura, S Tanaka, M Ohki, S Takahashi, T Kawai. 1986. Endotoxin-inactivating activity in normal and pathological human blood samples.. https://doi.org/10.1128/iai.53.2.294-297.1986

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