Flow-force relationships in mitochondrial oxidative phosphorylation.

H Woelders; J Putters; K van Dam

doi:10.1016/0014-5793(86)81379-5

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Flow-force relationships in mitochondrial oxidative phosphorylation.

H Woelders·J Putters·K van Dam

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Abstract

The rates of oxidation and phosphorylation in isolated rat-liver mitochondria have a steep dependence on the protonmotive force (delta mu H+) across the membrane. These experimentally observed relationships proved to be independent of the way in which delta mu H+ was varied. These results were obtained when the membrane potential (delta psi) was calculated from the distribution of K+ (in the presence of valinomycin). When triphenylmethylphosphonium (TPMP+) was used as a probe for delta psi, slightly different flow-force relationships were obtained. We conclude that unique relationships exist between delta mu H+ and the rates of oxidation and phosphorylation, and that under some conditions the behaviour of the probe TPMP+ is anomalous.

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Animals Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Mitochondria, Liver Onium Compounds Oxidative Phosphorylation Oxygen Consumption Potassium Rats Triphenylmethyl Compounds Valinomycin Journal Article Research Support, Non-U.S. Gov't

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H Woelders, J Putters, K van Dam. 1986-08-11. Flow-force relationships in mitochondrial oxidative phosphorylation.. https://doi.org/10.1016/0014-5793(86)81379-5

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