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Rapid bleach for melanin.

G Frangioni·G Borgioli
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Abstract

Complete bleaching of melanin in strongly pigmented specimens embedded in paraffin or polystyrene, and sectioned and mounted on slides, is possible in 1-3 hr at 37 C in a solution of 20 ml of benzyl alcohol, 10 ml of acetone, 5 ml of 10% hydrogen peroxide and 4 drops of a 25% ammonia solution. The bleached tissues are well preserved and tolerate further histochemical treatments. All the stains and reactions tested give results identical to or better than those obtained after 24-48 hr oxidation in 10% hydrogen peroxide.

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AnimalsHistocytochemistryMelaninsOxidation-ReductionJournal Article

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G Frangioni, G Borgioli. 1986. Rapid bleach for melanin.. https://doi.org/10.3109/10520298609109944

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