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A combined testing protocol approach for mutagenicity testing.

T H Connor·J Meyne·L Molina·M S Legator
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Abstract

The antischistosomal agent, hycanthone methanesulfonate (HMS), was employed to illustrate the utility of carrying out several mutagenicity tests in a single concurrent animal experiment. Several commonly used procedures that were successfully integrated into a multiple testing protocol included (1) metaphase analysis in bone marrow, (2) micronucleus test in bone marrow, (3) analysis of the urine for mutagenic constituents, and (4) the host-mediated assay using Salmonella typhimurium. In addition to these animal studies, in vitro mutagenicity testing with and without activation was carried out using S. typhimurium. HMS produced positive, dose--response effects in in vitro tests, metaphase analysis, micronucleus test, and urine analysis, but not in the host-mediated assay. The results of these integrated techniques suggest that such a protocol may be a benefit to those concerned with mutagenicity testing of chemicals.

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AnimalsCell NucleusChromosome AberrationsDrug Evaluation, PreclinicalFemaleGenetic TechniquesHycanthoneMiceMutagensSalmonella typhimuriumThioxanthenesComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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T H Connor, J Meyne, L Molina, M S Legator. 1979. A combined testing protocol approach for mutagenicity testing.. https://doi.org/10.1016/0165-1161(79)90132-8

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