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Platelet-activating factor in normal rat uterus.

K Yasuda·K Satouchi·K Saito
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Abstract

Platelet-activating factor (PAF) was found in normal rat uterus and identified as 1-0-hexadecyl/octadecenyl-2-acetyl-sn-glycero-3-phosphocholine. PAF was purified by several successive chromatographic procedures. It showed platelet aggregating activity, which was inhibited by CV 3988, and had no effect on platelets desensitized with 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine. The tert-butyldimethyl-silylderivative of 1-0-alkyl-2-acetyl-sn-glycerol, which was obtained by hydrolysis of uterine PAF with phospholipase C, was analyzed by gas chromatography-mass spectrometry. One rat uterus contained approximately 21.3 ng of 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine. This is the first report of the occurrence of a significant amount of PAF in a normal animal tissue.

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AnimalsFatty AlcoholsFemaleGas Chromatography-Mass SpectrometryPlasmalogensPlatelet Activating FactorPlatelet AggregationRatsUterusJournal ArticleResearch Support, Non-U.S. Gov't

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K Yasuda, K Satouchi, K Saito. 1986-08-14. Platelet-activating factor in normal rat uterus.. https://doi.org/10.1016/s0006-291x(86)80414-4

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