A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.

S Tanaka; H Fujimoto

doi:10.1016/0006-291x(86)90504-8

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A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.

S Tanaka·H Fujimoto

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Abstract

We have analysed by the DNA sequencing one of the cDNA clones, pPM459, to mRNA abundant in spermatids of mice. This clone contained 535 base pair nucleotides with a coding region for 139 amino acids and a 3' untranslated region including a single polyadenylation signal. Screening of the protein database revealed that the deduced amino acid sequence highly matched the carboxyl terminal residues 192-330 of mouse lactate dehydrogenase isozyme X (LDH-X). Taken together with our previous report which showed transcription of the message hybridizing to pPM459 after meiosis, it was demonstrated that LDH-X mRNA synthesis continued during the postmeiotic phase in spermatogenesis.

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Amino Acid Sequence Animals Base Sequence DNA DNA, Recombinant Isoenzymes L-Lactate Dehydrogenase Male Meiosis Mice Nucleic Acid Hybridization RNA Processing, Post-Transcriptional RNA, Messenger Spermatids Spermatogenesis Testis Transcription, Genetic Journal Article

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S Tanaka, H Fujimoto. 1986-04-29. A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.. https://doi.org/10.1016/0006-291x(86)90504-8

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