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An autoradiographic microtechnique to study lymphocyte stimulation in solid cultures.

E Kondracki
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Abstract

A microtechnique to study lymphocyte stimulation in solid cultures by autoradiography is described. Using this technique at a concentration of 2 x 10(6) lymphocytes/chamber, it was found that the percentage of proliferating cells was 8.4% with anti-lymphocyte serum, 3.9% with calcium ionophore A23187, 2.5% with phytohemagglutinin P, 5.8% with phytohemagglutinin HA17 and 4.2% with tetradecanoyl-phorbolacetate. Lymphocyte responses were dependent on cell concentration. Lymphocyte responses to phytohemagglutinins P and HA17 were potentiated by incorporation into the cultures of 5% autologous erythrocytes.

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AnimalsAntilymphocyte SerumAutoradiographyCalcimycinCells, CulturedHumansLymphocyte ActivationLymphocytesMitogensPhytohemagglutininsRabbitsJournal Article

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E Kondracki. 1979. An autoradiographic microtechnique to study lymphocyte stimulation in solid cultures.. https://doi.org/10.1016/0022-1759(79)90251-5

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