[Micronucleus test in cultured cells--preliminary study].

S J Cheng

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PubMed · 3769749

[Micronucleus test in cultured cells--preliminary study].

S J Cheng

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Abstract

In mutagenicity and carcinogenicity assays using micronucleus test, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 50 mg/kg or benzo(a)pyrene (BaP), 100 mg/kg did not increase the incidence of micronuclei in polychromatic erythrocytes in bone marrow of mice. MNNG, 0.1 microgram/ml could induce a significant increase of micronuclei with dose response in cultured V79 cells. In another experiment of Syrian hamster embryo cells, the induction of micronuclei was seen after benzo(a)pyrene, 0.1 microgram/ml treatment. The combined use of micronucleus test in vivo and in vitro can increase the sensitivity and reduce the false negativity obtained in vivo micronucleus test.

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Animals Benzo(a)pyrene Bone Marrow Cells, Cultured Cricetinae Ethyl Methanesulfonate Male Mesocricetus Methylnitronitrosoguanidine Mice Mutagenicity Tests English Abstract Journal Article

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S J Cheng. 1986. [Micronucleus test in cultured cells--preliminary study].. https://pubmed.ncbi.nlm.nih.gov/3769749/

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