Magnetic carbohydrate nanoparticles for affinity cell separation.

U Schröder; S Segrén; C Gemmefors; G Hedlund; B Jansson; H O Sjögren; C A Borrebaeck

doi:10.1016/0022-1759(86)90431-x

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Magnetic carbohydrate nanoparticles for affinity cell separation.

U Schröder·S Segrén·C Gemmefors·G Hedlund·B Jansson·H O Sjögren·C A Borrebaeck

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Abstract

Magnetically responsive nanoparticles were prepared from enzymatically hydrolysed starch and magnetite. Two different monoclonal antibodies were covalently coupled to the particles. The antibody-coupled particles were in the size range of 100-300 nm and had an iron content of about 60%. Using 100 micrograms of magnetic particles (coupled with monoclonal mouse anti-rat Ig kappa light chain antibody) a very high depletion of surface Ig positive cells (mostly B-cells) from one million rat peripheral blood mononuclear cells could be achieved. The separation efficiency was evaluated by flow cytofluorometric analysis. This technique permits the detection of a small number of surface Ig positive cells among 10,000 negative cells.

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Cell Separation Dextrins Flow Cytometry Lymphocytes Magnetics Receptors, Antigen, B-Cell Starch Journal Article Research Support, Non-U.S. Gov't

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U Schröder, S Segrén, C Gemmefors, G Hedlund, B Jansson, H O Sjögren, C A Borrebaeck. 1986-10-23. Magnetic carbohydrate nanoparticles for affinity cell separation.. https://doi.org/10.1016/0022-1759(86)90431-x

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