[Lambdoid phage structural proteins and antigens].

V N Ivanov; Iu L Andreev; S S Reshetnikov; N P Mertvetsov

PubMed · 379613

[Lambdoid phage structural proteins and antigens].

V N Ivanov·Iu L Andreev·S S Reshetnikov·N P Mertvetsov

Abstract

The composition of structural proteins of lambdoid phages such as lambda, phi 80 434 divided by molecular weights was determined by means of SDS-disc-electrophoresis in a 15% polyacrylamide gel. The proteins of the same phages were divided by isoelectric points using an isoelectric focusing in a 5,25% polyacrylamide gel with 8 M urea and a gradient pH 7.0--3.5. The both methods brought out a composition character of the virion proteins and illustrated the high degree of similarity among the structural proteins of phages lambda and 434 and a far less similarity among the proteins lambda and phi 80. The antigenic composition of the lambdoid phage was determined and the basic antigenes were identified on one-dimensional and two-dimensional immunoelectrophoregrams. The appreciable immunochemical affinity of basic antigenes of the lambda and 434, but a partial affinity of the phages lambda and phi 80 were found. The basic protein of the head pE proved to be immunochemically similar for all three phages.

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Antigens, Viral Coliphages Electrophoresis, Polyacrylamide Gel Escherichia coli Immunoelectrophoresis Molecular Weight Viral Proteins Journal Article

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V N Ivanov, Iu L Andreev, S S Reshetnikov, N P Mertvetsov. [Lambdoid phage structural proteins and antigens].. https://pubmed.ncbi.nlm.nih.gov/379613/

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