Experience with testing for mycoplasma with five different methods.

W Scheirer; H W Katinger; G Laber

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PubMed · 381077

Experience with testing for mycoplasma with five different methods.

W Scheirer·H W Katinger·G Laber

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Abstract

Five methods for mycoplasma-content detection in cell-cultures are established in this laboratory. Results with about 20 different cell lines continuously grown in this laboratory in some determinations indicate that four methods give good correspondence. A microbiological method gave contrary results in many cases. It is possible that new infections will grow on mycoplasma broth but the older infections are adapted to cell-culture and give negative results on artificial medium. New low-level infections cannot be detected by the other methods which do not include any efficient enrichment step. The most convenient method in our opinion is a DNA-staining method according to Chen because this is a very quick, inexpensive and easily performable process. In practice it seems necessary to check cell cultures in two ways, firstly with a quick method and additionally with the microbiological test to ensure the detection of new low-level infections.

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Animals Bacteriological Techniques Cell Line Cells, Cultured Humans Mycoplasma Comparative Study Journal Article

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W Scheirer, H W Katinger, G Laber. 1979. Experience with testing for mycoplasma with five different methods.. https://pubmed.ncbi.nlm.nih.gov/381077/

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