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Solid phase synthesis of thymosin beta 9.

N Chandramouli·K K Bhargava·G S Incefy·M J Modak·R B Merrifield
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Abstract

Thymosin beta 9, a 41 residue thymic polypeptide, has been synthesized by a solid phase method. A modification of the low HF method was used to deprotect and cleave the peptide from the resin. Thymosin beta 9 was then obtained in analytically pure form by a one-step purification procedure in 32% yield. The activity of thymosin beta 9 in the terminal deoxynucleotidyl transferase assay was greater than calf thymus fraction 5, but comparable to thymosin beta 4. In contrast to thymosin alpha 1, neither beta 4 nor beta 9 was active in the rosette inhibition assay.

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AnimalsDNA NucleotidylexotransferaseHydrocortisoneIndicators and ReagentsMiceMice, Inbred C57BLStructure-Activity RelationshipThymosinThymus GlandJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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N Chandramouli, K K Bhargava, G S Incefy, M J Modak, R B Merrifield. 1986. Solid phase synthesis of thymosin beta 9.. https://doi.org/10.1111/j.1399-3011.1986.tb03289.x

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