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Separation of opioid peptides utilizing high performance liquid chromatography.

R V Lewis·S Stein·S Udenfriend
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Abstract

Chromatographic procedures have been developed for resolving all of the known enkephalins and endorphins on a single column. The effect of eluant pH on the retention times and separation of the enkephalins and beta-endorphin was determined. By combining these separations with a sensitive radioreceptor assay it is possible to assay all of the opioid peptides in the pituitary gland or in various regions of the brain from individual small laboratory animals.

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Amino Acid SequenceAnimalsBrainChromatography, High Pressure LiquidEndorphinsHydrogen-Ion ConcentrationPituitary GlandRatsJournal Article

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R V Lewis, S Stein, S Udenfriend. 1979. Separation of opioid peptides utilizing high performance liquid chromatography.. https://doi.org/10.1111/j.1399-3011.1979.tb01911.x

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