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ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.

B M Gray
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Abstract

A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.

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AdsorptionAntigensEnzyme-Linked Immunosorbent AssayImmunoenzyme TechniquesMethodsPlasticsPolysaccharides, BacterialProteinsStreptococcus agalactiaeStreptococcus pneumoniaeJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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B M Gray. 1979. ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.. https://doi.org/10.1016/0022-1759(79)90340-5

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PubMed1979

The isoelectric point of thrombin and its behaviour compared to prothrombin at some solid surfaces.

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