Nuclear polyadenylate-binding protein.

A B Sachs; R D Kornberg

doi:10.1128/mcb.5.8.1993-1996.1985

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PubMed · 3837854

Nuclear polyadenylate-binding protein.

A B Sachs·R D Kornberg

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Abstract

Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously described. Similar results are obtained with yeast and mouse fibroblasts, indicating a high degree of conservation of both nuclear and cytoplasmic polyadenylate-binding proteins. The activity from rat liver nuclei has been purified 125-fold on the basis of specific binding to polyadenylate and shows two main bands in sodium dodecyl sulfate gels at 53 and 55 kilodaltons.

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Animals Carrier Proteins Cell Nucleus Electrophoresis, Polyacrylamide Gel Kinetics Liver Molecular Weight Poly(A)-Binding Proteins Rats Journal Article Research Support, U.S. Gov't, P.H.S.

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A B Sachs, R D Kornberg. 1985. Nuclear polyadenylate-binding protein.. https://doi.org/10.1128/mcb.5.8.1993-1996.1985

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