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Plasmodium falciparum in culture: improved continuous flow method.

W Trager
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Abstract

A new design of flow vessel provides a method for continuous culture of P. falciparum in a settled layer of human erythrocytes with a slow flow of culture medium over them. The parasitemia is kept fluctuating from approximately 1%, just after addition of fresh erythrocytes, to approximately 10%, 2 or 3 days later. Each vessel provides each week 3 harvests, each containing approximately 0.6--1 X 10(9) parasites.

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AnimalsCell DivisionCulture MediaCulture TechniquesErythrocytesGlucoseHumansPlasmodium falciparumJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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W Trager. 1979. Plasmodium falciparum in culture: improved continuous flow method.. https://doi.org/10.1111/j.1550-7408.1979.tb02747.x

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