[Binding of activated C3b component with complement effector].

L V Kozlov; L I Rostovtseva; T S Lomaka; N S Sutovskaia

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PubMed · 3849970

[Binding of activated C3b component with complement effector].

L V Kozlov·L I Rostovtseva·T S Lomaka·N S Sutovskaia

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Abstract

Various nucleophilic agents (acceptors) react with thiolester group of nascent activated fragment (C3b) of the third complement component. The C3b-acceptors binding prevents transformation of C3 convertase to C5 convertase and results in inhibition of the cell-target lysis. A convenient method of monitoring the EAC142 to EAC1423 transformation was elaborated. Character of the inhibition suggests that the covalent binding follows a stage of the reversible C3b-acceptor complex formation. The method allows to determine the maximum of inhibition of the C5 convertase formation and the dissociation constant of the reversible C3b-acceptor complex, which reflects the C3b affinity to this acceptor.

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Animals Buffers Complement Activating Enzymes Complement C3-C5 Convertases Complement C3b Erythrocytes Hemolysis In Vitro Techniques Kinetics Receptors, Complement Sheep English Abstract Journal Article

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L V Kozlov, L I Rostovtseva, T S Lomaka, N S Sutovskaia. 1985. [Binding of activated C3b component with complement effector].. https://pubmed.ncbi.nlm.nih.gov/3849970/

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