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PubMed · 3860836

A partial cDNA clone for human apolipoprotein B.

S S Deeb·A G Motulsky·J J Albers
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Abstract

A human liver cDNA library was screened for sequences coding for apolipoprotein B (apo B), the major protein of human low density lipoproteins. A mixture of synthetic oligonucleotides (26 bases long) coding for an amino acid sequence known to exist in apo B was used as a hybridization probe. A clone was identified that had a cDNA insert of 593 base pairs and that contained sequences coding for a peptide of 24 residues that had earlier been isolated from apo B by limited proteolysis. The entire nucleotide sequence of the cDNA insert consists of one open reading frame coding for 197 amino acids. Apo B-related RNAs were found in human liver, baboon liver, and the human hepatoma cell line HepG2. None were detected in placenta, simian virus 40 (SV40)-transformed fibroblasts, and a lymphoblastoid cell line. The length of the mature apo B mRNA was estimated to be 18 kb, enough to code for a protein with a molecular weight in the neighborhood of 500,000.

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Amino Acid SequenceAnimalsApolipoproteins BBase SequenceCloning, MolecularDNAHumansLiverRNA, MessengerComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S S Deeb, A G Motulsky, J J Albers. 1985. A partial cDNA clone for human apolipoprotein B.. https://doi.org/10.1073/pnas.82.15.4983

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