Two B-cell subpopulations identified by flow cytometry.

J R Watkins; M R Loken; K L Knight

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PubMed · 3876983

Two B-cell subpopulations identified by flow cytometry.

J R Watkins·M R Loken·K L Knight

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Abstract

Ig+ spleen cells were analysed by light scatter analysis on a flow cytometer and two distinct subpopulations were identified. The large Ig+ cells (10-11 micron in diameter) were found in spleen and bone marrow, whereas the small Ig+ cells (7-8 micron in diameter) were found in all lymphoid tissues. Of the total Ig+ splenic lymphocytes, 40-60% were large Ig+ cells and had surface IgM, Ia and Fc gamma receptors. The large Ig+ cells were highly enriched for responsiveness to the B-cell mitogens, anti-Ig, Nocardia water-soluble mitogen and LPS, whereas the small Ig+ splenic cells had little or no responsiveness to these mitogens.

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Animals B-Lymphocytes Cell Separation Flow Cytometry Histocompatibility Antigens Class II Immunoglobulin M Mitosis Rabbits Receptors, Antigen, B-Cell Receptors, Fc Spleen Tissue Distribution Journal Article Research Support, U.S. Gov't, P.H.S.

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J R Watkins, M R Loken, K L Knight. 1985. Two B-cell subpopulations identified by flow cytometry.. https://pubmed.ncbi.nlm.nih.gov/3876983/

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