A simple method for high resolution light microscopy of nervous tissue.

M P Pender

doi:10.1016/0165-0270(85)90100-1

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A simple method for high resolution light microscopy of nervous tissue.

M P Pender

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Abstract

A simple method for high resolution light microscopy of nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in HistoResin (LKB Bromma), a new glycol methacrylate based embedding medium, and sectioned (2 microns) on a standard microtome. The method gives excellent preservation of tissue structure, which is far superior to that in paraffin sections and in fact resembles that in epoxy resin-embedded material. It does not, however, require the time, expertise and ultramicrotome needed to cut epoxy resin sections. This technique is particularly useful for assessing demyelination.

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Animals Benzoxazines Encephalomyelitis, Autoimmune, Experimental Methacrylates Microscopy Microtomy Nerve Tissue Oxazines Rats Rats, Inbred Lew Tolonium Chloride Journal Article Research Support, Non-U.S. Gov't

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M P Pender. A simple method for high resolution light microscopy of nervous tissue.. https://doi.org/10.1016/0165-0270(85)90100-1

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