PubMed · 388603
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
C Van Ypersele de Strihou. 1979-04-21. [Urinary sediment].. https://pubmed.ncbi.nlm.nih.gov/388603/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Explore the source record for details and available documents.
The efficacy of sedimentation combined with cytocentrifugation of sputa was examined by preparing smears for the detection of mycobacteria. The detection of acid-fast bacilli from sputum sediment by cytocentrifugation yielded 32 smears positive for acid-fast bacilli, out of which in 26 cases better results were obtained than by the conventional method. More bacilli were found in 20 cases and on 6 occasions solely the cytospin method was positive. The differences in the number of bacilli were 100-200-fold in 5 cases and 10-fold in 12 paired cases. The technique applied is suitable for making culture and smear from the same sediment. The sedimentation combined with cytocentrifugation increases the effectiveness of the detection of acid-fast bacilli in smears.
PURPOSE: The main goal was to establish that sedimentation field-flow fractionation (SdFFF), operated with power based field programming, is effective in the characterization of a commercial emulsion, Medialipide. This emulsion is used clinically for total parenteral nutrition and it is consisted of a mixture of long-chain triglycerides (LCT, soybean oil) with medium-chain triglycerides (MCT) emulsified by phospholipids. METHODS: Different field programming methods were used in the analysis to establish the limits of applicability of the technique. RESULTS: Identical size distribution profiles were obtained under various conditions of the analysis. The density of the droplets was determined by collecting fractions from the SdFFF eluting bands, and analyzing them by photon correlation spectroscopy. The value of density of the oil droplets was changed in the SdFFF data, until best agreement with the PCS values was achieved. The value of density corresponding to the best agreement was considered as the oil density, and it was closed to the weighted average value between soybean and MCT oils. CONCLUSIONS: Field programming extends the capabilities of sedimentation field-flow fractionation in handling and characterizing complex and delicate samples as Medialipide.