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PubMed · 3901840

Bovine neutrophils treated with chemotactic agents: morphologic changes.

J H Forsell·J R Kateley·C W Smith
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Abstract

Neutrophils were isolated from the peripheral blood of cattle. After the neutrophils were incubated with zymosan-activated serum, the neutrophils changed from spherical to a bipolar shape. Ninety percent of the neutrophils became bipolar in 5 to 10 minutes. Bacterial cell filtrate and casein also induced bipolar shape changes in neutrophils, but N-formyl-L-methionyl-L-leucinyl-L-phenylalanine did not. The neutrophil-shape-change response was a rapid in vitro assay to evaluate early chemotactic events.

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AnimalsCaseinsCattleCulture MediaEosinophilsEscherichia coliFemaleLactationMicroscopy, Phase-ContrastN-Formylmethionine Leucyl-PhenylalanineNeutrophilsPregnancyComparative StudyJournal Article

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J H Forsell, J R Kateley, C W Smith. 1985. Bovine neutrophils treated with chemotactic agents: morphologic changes.. https://pubmed.ncbi.nlm.nih.gov/3901840/

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