Microtest for red cell typing.

Abstract

A simple microtest for red cell (RBC) typing was developed which required 0.002 ml of reagents per test. After mixing RBCs with antibody, the microtray was incubated upside down for 10 to 15 minutes at 37 degrees C, and 10 minutes at room temperature. The trays were read after reinversion and allowing 10 to 15 minutes for the RBCs to settle. Antibodies must be selected that react under these conditions. Two examples each of anti-A, -B, -C, -D, -E, -c, -M, and -N were tested against a panel of 500 individuals. The results were generally concordant with each other and with the conventional tube test results. The procedure is simple to perform and involves minimal costs for reagent antisera.