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PubMed · 3928486

Decrease of HLA-DR antigen expression by human monocytes during cultivation in absence of exogenous or endogenous interferon-gamma.

H D Volk·S R Waschke·W Diezel·R Grunow·R von Baehr·H Fiebig
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Abstract

The kinetics of HLA-DR antigen expression by human peripheral blood monocytes during cultivation in vitro was studied. Immediately after separation by glass adherence, about 60% of monocytes expressed DR antigens as judged by indirect fluorescence staining with a monoclonal antibody (BL-DR/1). Monocytes carefully depleted of lymphocytes, gradually lost their DR antigens during cultivation in the absence of exogenous interferon-gamma (IFN-gamma). However, addition of a few lymphocytes to the adherent cells prevented the decrease of DR antigen expression. Furthermore, it was shown that doses as low as 1 IU/ml of IFN-gamma are sufficient to induce DR antigen expression by cultured monocytes. Experiments with cyclosporin A suggest that lymphocytes contaminating the monocyte preparations can produce spontaneously sufficient amounts of IFN-gamma for maintenance of the DR antigens on monocytes.

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Cells, CulturedCyclosporinsHLA-DR AntigensHistocompatibility Antigens Class IIHumansInterferon-gammaKineticsMonocytesJournal Article

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BibTeXRIS

H D Volk, S R Waschke, W Diezel, R Grunow, R von Baehr, H Fiebig. 1985. Decrease of HLA-DR antigen expression by human monocytes during cultivation in absence of exogenous or endogenous interferon-gamma.. https://doi.org/10.1016/0165-2478(85)90184-1

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Cells, Cultured↗