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Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics.

Guijie Zhu·Seyed Amirhossein Sadeghi·Morteza Mahmoudi·Liangliang Sun
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Abstract

The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine.

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ProteomicsMass SpectrometryIsoelectric FocusingProtein CoronaNanoparticlesHumansCapillary Isoelectric FocusingJournal Article

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Guijie Zhu, Seyed Amirhossein Sadeghi, Morteza Mahmoudi, Liangliang Sun. 2024-10-08. Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics.. https://doi.org/10.1039/d4cc02666g

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