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Competence proteins in Bacillus subtilis com mutants.

C Barberio·R Coppolecchia·G Mastromei·M Polsinelli
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Abstract

The synthesis of nucleases and proteins specific for competence development have been studied in four different Bacillus subtilis competence-deficient mutants. The nuclease analysis showed that two DNA-binding-deficient mutants were impaired in three nuclease activities involved in binding and entry of donor DNA. The other two strains did not show any reduction in nuclease activities. Two-dimensional gel electrophoresis of the proteins, synthesized during competence development, revealed that all four mutants are lacking several competence-specific polypeptides. Our data show that these com mutations have a strong pleiotropic effect, which could be due to a block in the metabolic pathway leading to competence development.

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Bacillus subtilisBacterial ProteinsCell MembraneDNA-Binding ProteinsDeoxyribonucleasesElectrophoresis, Polyacrylamide GelMutationJournal Article

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C Barberio, R Coppolecchia, G Mastromei, M Polsinelli. 1985-10-17. Competence proteins in Bacillus subtilis com mutants.. https://doi.org/10.1016/0304-4165(85)90201-6

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