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PubMed · 3935005

Chromogenic substrates for sulfurtransferases.

M R Burrous·J Westley
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Abstract

The azo dye 4-(dimethylamino)-4'-azobenzene (DAB) thiosulfonate anion can serve as a sulfur-donor substrate for rhodanese (thiosulfate: cyanide sulfurtransferase, EC 2.8.1.1) and for thiosulfate reductase (EC unassigned) with cyanide anion and GSH, respectively, as acceptor substrates. In either case, the dye product is DAB sulfinate, which differs substantially in light absorption at 500 nm. Moreover, DAB sulfinate can serve as a sulfur-acceptor substrate for rhodanese with either inorganic thiosulfate or a colorless thiosulfonate anion as donor, and this reaction provides a second chromogenic assay procedure.

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Azo CompoundsCatalysisChromogenic CompoundsOxidoreductasesOxidoreductases Acting on Sulfur Group DonorsSpectrophotometrySubstrate SpecificitySulfurtransferasesThiosulfate Sulfurtransferasep-DimethylaminoazobenzeneJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M R Burrous, J Westley. 1985-08-15. Chromogenic substrates for sulfurtransferases.. https://doi.org/10.1016/0003-2697(85)90477-4

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