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PubMed · 3947372

Sequence specific antibodies to a deoxyribodinucleotide.

S C Chandrasekharappa·A S Gopalakrishnan·T M Jacob
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Abstract

Antibodies were elicited in rabbits to the dinucleotide, dpTpA, conjugated to a carrier protein. Among a few deoxyribomono- and oligonucleotides tested for binding to the antibodies by nitrocellulose tilter binding assay, only dpTpA and dpTpA containing oligonucleotides showed binding. The inhibition of the binding of 3H-dpTpA to the antibodies by various nonradioactive mono- and oligonucleotides showed that the antibodies were sequence specific and recognized the whole molecule of dpTpA. dpTpA specific antibodies were purified by a two step affinity chromatography. By competition studies, it was found that the purified antibodies bound denatured DNA at dpTpA sequences. The antibodies did not bind RNA.

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AntibodiesAntigen-Antibody ComplexCross ReactionsOligodeoxyribonucleotidesOligonucleotidesSerum Albumin, BovineStructure-Activity RelationshipComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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S C Chandrasekharappa, A S Gopalakrishnan, T M Jacob. 1986. Sequence specific antibodies to a deoxyribodinucleotide.. https://pubmed.ncbi.nlm.nih.gov/3947372/

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