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PubMed · 3948387

Practical "stat" method for radioimmunoassay.

S J Cembal
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Abstract

A prompt and reasonably precise radioimmunoassay result is often urgently required, but it is difficult to satisfy such requests without running a complete standard curve, even when only a single sample is to be assayed. To obtain a greater constancy in the response of the RIA system, I propose a method for use of RIA in a "stat" (i.e., urgent) method: maximum binding and nonspecific binding for RIA data processing are "calculated" from response indexes obtained in a previous assay run with the same reagents and from the binding values of two or three standards processed together with a single sample. These calculated values are then used to calculate the response index for the single sample, which is directly interpolated in the calibration curve of the previous non-stat assay. I checked the validity of this stat method for eight analytes, comparing a total of 552 analytical results obtained by the conventional and the stat method. This method is shown to give adequately precise results for use in clinical emergencies.

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HumansIndicators and ReagentsMathematicsPhenytoinRadioimmunoassayTime FactorsJournal Article

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S J Cembal. 1986. Practical "stat" method for radioimmunoassay.. https://pubmed.ncbi.nlm.nih.gov/3948387/

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