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Production of L-serine by Sarcina albida.

M Ema·T Kakimoto·I Chibata
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Abstract

Conditions for the production of microbial L-serine hydroxymethyltransferase and for the conversion of glycine to L-serine were studied. A number of microorganisms were screened for their abilities to form and accululate L-serine from glycine, and Sarcina albida was selected as the best organism. Enzyme activity in this organism as high as 0.12 U/ml could be produced in shaken cultures at 30 degrees C in a medium containing glucose, ammonium sulfate, glycine, yeast extract, and inorganic salts. L-Serine was produced most efficiently by shaking cells at 30 degrees C in a reaction mixture containing 20% glycine, 5 X 10(-3) M formaldehyde, and 3 X 10(-4) M pyridoxal phosphate in yields of 22 mg of broth in 5 days. L-Serine was easily isolated in 84% yields by ion-exchange resin.

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BacteriaCulture MediaGlycineGlycine HydroxymethyltransferaseHydrogen-Ion ConcentrationL-Serine DehydrataseSarcinaSerineSpecies SpecificityStereoisomerismTemperatureJournal Article

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M Ema, T Kakimoto, I Chibata. 1979. Production of L-serine by Sarcina albida.. https://doi.org/10.1128/aem.37.6.1053-1058.1979

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