Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.

Barbara Sivakova; Jan Jurcik; Veronika Lukacova; Laura Olivia Lalakova; Tomas Selicky; Ingrid Cipakova; Peter Barath; Lubos Cipak

doi:10.1007/978-1-0716-4168-2_21

PubMed · 39527209

Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.

Barbara Sivakova·Jan Jurcik·Veronika Lukacova·Laura Olivia Lalakova·Tomas Selicky·Ingrid Cipakova·Peter Barath·Lubos Cipak

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Abstract

Protein phosphorylation is a dynamic, reversible posttranslational modification that plays an important role in the regulation of cell signaling. Recently, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. In this chapter, we describe how to apply LFQ phosphoproteomics that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies for identification and quantification of changes in the phosphoproteome in the fission yeast Schizosaccharomyces pombe.

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Schizosaccharomyces Proteomics Phosphoproteins Schizosaccharomyces pombe Proteins Phosphorylation Phosphopeptides Proteome Protein Processing, Post-Translational LFQ phosphoproteomics Mass spectrometry PGC fractionation Phosphopeptide enrichment SAX fractionation Schizosaccharomyces pombe Journal Article Research Support, Non-U.S. Gov't

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Barbara Sivakova, Jan Jurcik, Veronika Lukacova, Laura Olivia Lalakova, Tomas Selicky, Ingrid Cipakova, Peter Barath, Lubos Cipak. 2025. Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.. https://doi.org/10.1007/978-1-0716-4168-2_21

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