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PubMed · 39546201

RNA Sequencing Protocols for Short-Read Sequencing.

Laura Vasquez-Velez·Veera D'Mello·Patricia Soteropoulos
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Abstract

RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform. Here we describe four different library preparation protocols for short-read sequencing: cDNA synthesis with poly(A) selection, library preparation with ribosomal depletion, and cDNA synthesis with SMART® (Switching Mechanism at 5' end of RNA Template) technology for low and Pico inputs.

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Gene LibraryDNA, ComplementarySequence Analysis, RNAHigh-Throughput Nucleotide SequencingHumansRNALibrary preparationPoly(A) selectionRNA-seqRibosomal depletionShort-read sequencingcDNAJournal Article

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Laura Vasquez-Velez, Veera D'Mello, Patricia Soteropoulos. 2025. RNA Sequencing Protocols for Short-Read Sequencing.. https://doi.org/10.1007/978-1-0716-4192-7_8

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