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HiChIP for Plant Tissues.

Rim Brik Chaouche·Cécile Raynaud·Moussa Benhamed·David Latrasse
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Abstract

While most epigenomics studies are based on a linear view of genome organization, the necessity to take the three-dimensional chromatin folding into account to understand transcriptional regulation is now clearly recognized. In the past years, approaches combining proximity-based ligation with high-throughput sequencing have opened the way to study long/short-range chromatin interactions and, thus, to analyze 3D chromatin organization. Among them, HiChIP, a protein-based method to capture chromatin interactions, gave rise to the most comprehensive view of the chromatin contacts involving specific chromatin components in a given system. Here, we describe a detailed procedure to produce HiChIP libraries starting from plant tissues.

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ChromatinPlantsHigh-Throughput Nucleotide SequencingEpigenomicsGene LibraryChromatin conformationHiChIPJournal ArticleResearch Support, Non-U.S. Gov't

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Rim Brik Chaouche, Cécile Raynaud, Moussa Benhamed, David Latrasse. 2025. HiChIP for Plant Tissues.. https://doi.org/10.1007/978-1-0716-4228-3_3

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