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Quantification of tryptamine in brain using high-performance liquid chromatography.

A A Larson·N L Dalo
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Abstract

The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a muBondapak C18 reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 +/- 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, tranylcypromine and l-tryptophan increased the concentration to 96.7 +/- 21.9 ng/g.

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AcetylationAnimalsBrain ChemistryChromatography, High Pressure LiquidMaleRatsRats, Inbred StrainsSolventsSpectrometry, FluorescenceTryptaminesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A A Larson, N L Dalo. 1986-02-14. Quantification of tryptamine in brain using high-performance liquid chromatography.. https://doi.org/10.1016/s0378-4347(00)83689-0

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