Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout.

Esther Feng Ying Ng; Franz Meitinger

doi:10.1007/978-1-0716-4224-5_12

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Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout.

Esther Feng Ying Ng·Franz Meitinger

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Abstract

Genetic engineering and screening in human cells are powerful techniques for the precise and comprehensive identification and analysis of gene and protein domain functions. Genome-wide knockout screens have been extensively utilized to discover essential genes, tumor suppressors, and genes that regulate responses to various chemicals, including antimitotic and therapeutic drugs. The advent of base editors, which facilitate the targeted mutation of single amino acids, has advanced the identification of critical and functional domains or motifs. In this context, we outline methods for creating efficient base editor and inducible knockout cell lines for targeted gene manipulation and conducting genetic screens to elucidate the roles of genes and their domains within a specific cell biological context.

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Humans Gene Editing Gene Knockout Techniques CRISPR-Cas Systems Genetic Engineering Genetic Testing Base editing CRISPR Cas9 Gene editing Gene engineering Gene knockout Genetic screening Journal Article Research Support, Non-U.S. Gov't

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Esther Feng Ying Ng, Franz Meitinger. 2025. Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout.. https://doi.org/10.1007/978-1-0716-4224-5_12

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