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PubMed · 3964819

The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization.

J Tschopp·E R Podack·H J Müller-Eberhard
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Abstract

Polymerization of C9 occurs spontaneously or can be induced by the tetramolecular complex C5b-8. Spontaneous C9 (0.15 mg/ml) polymerization required more than 3 days at 37 degrees C. In the presence of C5b-8, C9 polymerization was complete within 10 min. The molar C9:C5b-8 ratio determined the extent of tubular poly C9 formation by C5b-8-bearing phospholipid vesicles. When this ratio was 9:1 or 12:1, 72% of complex-bound C9 was present as SDS resistant tubular poly C9 (Mr = 1.1 X 10(6]. At lower C9:C5b-8 ratios, poly C9 was bound primarily in nontubular form. Tubular poly C9, as part of C5b-9, could also be generated on rabbit erythrocytes by using whole human serum as a complement source. At limiting serum concentration (molar C9 to C8 ratio approximately 2), no SDS-resistant tubular poly C9 was detected. At high serum concentration or when using serum that was supplemented with C9, up to 40% of the C9 was SDS-resistant tubular poly C9, and the rest was poly C9, which was incompletely polymerized. It is suggested that the C5b-8 complex acts as an accelerator of C9 polymerization, and that its relative concentration to C9 determines the ultrastructure of the C5b-9 complex.

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AnimalsComplement C9Complement System ProteinsElectrophoresis, Polyacrylamide GelErythrocyte MembraneHemolysisHumansKineticsMacromolecular SubstancesMolecular WeightRabbitsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J Tschopp, E R Podack, H J Müller-Eberhard. 1985. The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization.. https://pubmed.ncbi.nlm.nih.gov/3964819/

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