Capturing Chromatin Organization by MNase-seq and ATAC-seq.

Mika Saotome; Jill Goodman; Motoki Takaku

doi:10.1007/978-1-0716-4322-8_12

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Capturing Chromatin Organization by MNase-seq and ATAC-seq.

Mika Saotome·Jill Goodman·Motoki Takaku

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Abstract

Hox genes play a pivotal role during development. Their expression is tightly controlled in a spatiotemporal manner, ensuring that specific body structures develop at the correct locations and times during development. Various genomics approaches have been used to capture temporal and dynamic regulation of Hox gene expression at the nucleosome/chromatin level. This chapter focuses on the utilization of capture MNase-seq and Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq), two advanced techniques that enable the exploration of chromatin accessibility and nucleosome positioning within these critical genomic regions.

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Chromatin Nucleosomes Animals Chromatin Immunoprecipitation Sequencing Transposases High-Throughput Nucleotide Sequencing Micrococcal Nuclease Humans Sequence Analysis, DNA Chromatin Assembly and Disassembly ATAC-seq Epigenetics Gene regulation MNase-seq Journal Article Research Support, N.I.H., Extramural

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Mika Saotome, Jill Goodman, Motoki Takaku. 2025. Capturing Chromatin Organization by MNase-seq and ATAC-seq.. https://doi.org/10.1007/978-1-0716-4322-8_12

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