Paired Single-Cell Transcriptome and DNA Barcode Detection in Zebrafish Using ScarTrace.

Chlo&#xe9; S Baron; Anna Alemany

doi:10.1007/978-1-0716-4310-5_11

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Paired Single-Cell Transcriptome and DNA Barcode Detection in Zebrafish Using ScarTrace.

Chloé S Baron·Anna Alemany

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Abstract

ScarTrace is a CRISPR/Cas9-based genetic lineage tracing method that allows for uniquely barcoding the DNA of single cells at a target GFP sequence during developing zebrafish embryos. Single cells from barcoded adult zebrafish can be isolated from various tissues (e.g., marrow, brain, eyes, fins), and their transcriptome and barcode sequences are captured by single-cell cDNA amplification and genomic DNA nested PCR, respectively. Computationally, cell type and barcode identification permit clone tracing and lineage tree reconstruction of tissues to unravel fate decisions during embryogenesis.

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Animals Zebrafish Single-Cell Analysis DNA Barcoding, Taxonomic CRISPR-Cas Systems Transcriptome Gene Expression Profiling Embryo, Nonmammalian CRISPR/Cas9 Clone tracing Genetic lineage tracing Lineage tree reconstruction Next-generation sequencing Single-cell barcode capture Single-cell transcriptomics Zebrafish development Journal Article

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Chloé S Baron, Anna Alemany. 2025. Paired Single-Cell Transcriptome and DNA Barcode Detection in Zebrafish Using ScarTrace.. https://doi.org/10.1007/978-1-0716-4310-5_11

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