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A high-precision automatic closed-circuit respirometer for small animals.

R J Howland·K Newman
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Abstract

An automatic apparatus for the continuous measurement of O2 consumption of small laboratory animals is described. By use of a high-sensitivity pressure transducer with associated circuitry together with a peristaltic O2 delivery system, the closed respirometer chamber is maintained at atmospheric pressure +/- 0.5 mmH2O. O2 delivery is measured to within 0.25 ml by recording rotations of the peristaltic pump, following calibration by the withdrawal of a preset volume of air from the chamber. Static trials (with the chamber empty) indicate a high degree of reproducibility of data with the chamber pressure remaining at atmospheric pressure +/- 0.5 mmH2O as a result of the proportional, as opposed to fixed-volume, delivery of O2. Trials with mice and rats have likewise produced data with a high degree of reproducibility.

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AnimalsAnimals, LaboratoryAtmosphere Exposure ChambersCalibrationRespiratory Function TestsTransducers, PressureJournal Article

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BibTeXRIS

R J Howland, K Newman. 1985. A high-precision automatic closed-circuit respirometer for small animals.. https://doi.org/10.1152/jappl.1985.58.3.1031

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