PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 3988349

Recombination-deficient Streptococcus sanguis.

L Daneo-Moore·A Volpe
View original source ↗Export metadata ↓

Abstract

A UV-sensitive derivative was obtained from Streptococcus sanguis Challis. The organism could be transformed with a number of small streptococcal plasmids at frequencies equal to, or 1 logarithm below, the transformation frequencies for the parent organism. However, transformation with chromosomal DNA was greatly impaired in the UV-sensitive derivative.

Explore related subjects

Methyl MethanesulfonateMutationPlasmidsRecombination, GeneticStreptococcus sanguisTransformation, BacterialUltraviolet RaysJournal ArticleResearch Support, U.S. Gov't, P.H.S.

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

L Daneo-Moore, A Volpe. 1985. Recombination-deficient Streptococcus sanguis.. https://doi.org/10.1128/iai.48.2.584-586.1985

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1979

Isolation of a recombination deficient Agrobacterium tumefaciens mutant.

The isolation of a recombination deficient (Rec-) strain of Agrobacterium tumefaciens is described. Strain LBA 4011 was mutagenized with nitrosoguanidine and after segregation 18,000 colonies were replica plated and UV irradiated. Twentytwo UV sensitive strains were isolated and tested for methylmethanesulphonate (MMS) sensitivity. Six of these strains were more MMS-sensitive than LBA 4011. A Ti plasmid that was genetically marked with Tn 1 (CbR) was introduced in these strains and the rescue of the CbR marker during superinfection with an incompatible cointegrate plasmid Ti::R 702 was determined. One strain exhibited a large reduction in rescue frequency. It is concluded that the latter strain was recombination deficient. This property did not influence the induction of plant tumours.

Methyl Methanesulfonate↗