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Temporal stability and lack of variance in microbiome composition and functionality in fit recreational athletes.

Ciara M O' Donovan·Sai Ravi Chandra Nori·Fergus Shanahan·Gerardina Celentano·Thomas Brendan Murphy·Paul D Cotter·Orla O' Sullivan
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Abstract

Human gut microbiome composition and function is influenced by environmental and lifestyle factors, including exercise and fitness. We studied the composition and functionality of the faecal microbiome of recreational (non-elite) runners (n = 62) with serial shotgun metagenomics, at 4 time points over a 7-week period. Gut microbiome composition and function was stable over time. Grouping of samples on the basis of their fitness level (fair, good, excellent, and superior) or habitual training (low (4-6 h/week), medium (7-9 h/week), high (10-12 h/week), and extreme (13 + hours/week)) revealed no significant microbiome-related differences. Overall, the species Faecalibacterium prausnitzii, Blautia wexlerae, and Prevotella copri were the most abundant members of the gut microbiome. Analysis of co-abundance groups (CAGs) revealed no significant relationship between CAGs and fitness levels or training subgroups. Functional pathways were similar across all samples and timepoints with no clustering based on associated metadata. The most abundant genes identified within samples corresponded to pathways for nucleoside and nucleotide biosynthesis, amino acid biosynthesis, and cell wall biosynthesis. Collectively, these results describe the microbiome of active recreational runners and note temporal stability amongst participants.

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HumansGastrointestinal MicrobiomeMaleFecesAdultFemaleAthletesMetagenomicsRunningYoung AdultMetagenomeFaecal microbiomeFitnessJournal ArticleResearch Support, Non-U.S. Gov't

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Ciara M O' Donovan, Sai Ravi Chandra Nori, Fergus Shanahan, Gerardina Celentano, Thomas Brendan Murphy, Paul D Cotter, Orla O' Sullivan. 2025-02-15. Temporal stability and lack of variance in microbiome composition and functionality in fit recreational athletes.. https://doi.org/10.1038/s41598-025-88723-9

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