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PubMed · 3996103

The structural basis for C-banding. A scanning electron microscopy study.

E M Jack·C J Harrison·T D Allen·R Harris
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Abstract

The same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.

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Azure StainsCentromereChromosome BandingChromosomesChromosomes, Human, 1-3Chromosomes, Human, 16-18Chromosomes, Human, 6-12 and XHeterochromatinHot TemperatureHumansMaleMicroscopy, Electron, ScanningPolymorphism, GeneticY ChromosomeJournal ArticleResearch Support, Non-U.S. Gov't

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E M Jack, C J Harrison, T D Allen, R Harris. 1985. The structural basis for C-banding. A scanning electron microscopy study.. https://doi.org/10.1007/bf00291009

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