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PubMed · 4004875

Platelet-associated IgG is a specific protein.

M Steiner
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Abstract

The immunoglobulin binding to normal human platelets (PaIgG) was isolated on cell columns in which platelets or their membranes were attached via concanavalin A to an inert support matrix. Normal human IgG isolated from pooled serum was applied to the cell columns. The absorbed material which was eluted at low pH with a buffer of high ionic strength was immunologically and biochemically pure IgG. When the nonadherent IgG of the first passage through the platelet cell column was reapplied a second time virtually no IgG was retained. Isoelectric focusing on urea SDS polyacrylamide gels revealed only 2 major bands with pIs of 8.2 and 8.4 whereas the precolumn IgG contained a wide range of molecular species with pIs ranging from less than 6.0 to 9.0.

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Blood PlateletsHumansHydrogen-Ion ConcentrationImmunoelectrophoresisImmunoglobulin GIsoelectric FocusingMolecular WeightOsmolar ConcentrationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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M Steiner. 1985-05-31. Platelet-associated IgG is a specific protein.. https://doi.org/10.1016/0006-291x(85)91423-8

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