Biochemical reconstitution of a type I-B CRISPR-associated transposon.

Shukun Wang; Leifu Chang

doi:10.1016/bs.mie.2025.01.042

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Biochemical reconstitution of a type I-B CRISPR-associated transposon.

Shukun Wang·Leifu Chang

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Abstract

CRISPR-associated transposons (CASTs) are potential gene editing tools because of their RNA-guided DNA insertion activity. It is essential to understand the mechanisms underlying the transposition for the application of CASTs. Here, we provide protocols for the biochemical reconstitution of a type I-B CAST for RNA-guided transposition. The procedures may be applicable to other types of CASTs and facilitate the mechanism studies of various CASTs.

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DNA Transposable Elements CRISPR-Cas Systems Gene Editing RNA, Guide, CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Biochemical reconstitution CRISPR CRISPR-associated transposon Protein purification Transposition assay Journal Article Research Support, N.I.H., Extramural

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Shukun Wang, Leifu Chang. 2025-02-26. Biochemical reconstitution of a type I-B CRISPR-associated transposon.. https://doi.org/10.1016/bs.mie.2025.01.042

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