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Complement activation by clumping factor and protein A from Staphylococcus aureus strain E 2371.

F Espersen
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Abstract

Purified surface components from Staphylococcus aureus strain E 2371, which bind to human plasma proteins, were tested for their ability in vitro to activate complement present in normal human serum. Staphylococcal clumping factor as well as protein A both isolated from strain E 2371 were capable of activate complement, while the fibronectin-binding protein showed no activity. At identical molar concentrations clumping factor was more potent than protein A to induce complement consumption. Both clumping factor and protein A were able to activate the alternative complement pathway as demonstrated in serum chelated with ethylene-glycerol-tetra-acetic acid (10 mM) in the presence of MgCl2 (10 mM).

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Chromatography, AffinityCoagulaseComplement ActivationHumansSepharoseStaphylococcal Protein AStaphylococcus aureusJournal ArticleResearch Support, Non-U.S. Gov't

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F Espersen. 1985. Complement activation by clumping factor and protein A from Staphylococcus aureus strain E 2371.. https://doi.org/10.1111/j.1699-0463.1985.tb02923.x

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