PubMed · 4017804
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G Hovorka. 1985. Tuning meter can replace oscilloscope for flow cytometer alignment.. https://doi.org/10.1002/cyto.990060418
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A gas chromatographic-mass spectrometric method was developed to determine eperisone hydrochloride, 4'-ethyl-2-methyl-3-piperidinopropiophenone hydrochloride, in human plasma over the concentration range 0.2-40 ng/ml. Excellent sensitivity was achieved by selection of a favorable fragment ion, m/z 98, of eperisone and reduction of heat decomposition of eperisone by using a splitless injector and a shortened capillary column. The method described here allows the determination of plasma concentrations as low as 0.2 ng/ml, the concentration attained 6 h after a single oral administration of 50 mg. At eperisone hydrochloride concentrations higher than 0.5 ng/ml, the mean inter-day variation of accuracy of the assay was less than 12%.
A method for the rapid quantitative analysis of dot blot assays is presented. A video camera, an NTSC compatible frame grabber board, and an AT personal computer are used to read photographic exposures of the assay plate. Image processing and image analysis techniques are used to calculate the orientation of the dot raster and then to compensate for the effect of variations in field illumination on measurements of local contrast. Local contrast (between dots and background) is an exponential function of the amount of hybridization between blotted DNA and complimentary oligonucleotide probes. The amount of hybridization between blotted DNA and oligonucleotide probes of known sequence is the criteria used to establish HLA-DR tissue types. Although the assay described here utilizes a chemiluminescent reaction, this algorithm may be used to read any assay that produces a rectangular raster of dots.
A high-performance liquid chromatographic method is described for the determination of terbutaline in human plasma in the range 1-35 ng/ml. Detection was achieved using a carbon fibre micro-electrochemical detector and a column-switching system. The microelectrode cell has advantages over conventional glassy carbon electrode-based detection systems in that it is easy to prepare, flexible in its operation and suffers less trouble from problems such as air bubbles and leaks. Furthermore, it has a better detection limit for terbutaline (0.8 ng/ml) to that obtained using a conventional glassy carbon electrode flow detector (2 ng/ml). Sample clean-up was by on-line solid-phase extraction with column switching, providing a method which was sensitive and reproducible, where the mean overall coefficient of variation was 5.60% and drug recovery in excess of 86% at the concentration levels studied.