Crystals of crotoxin suitable for high resolution x-ray diffraction analysis.

A Achari; F R Radvanyi; D Scott; C Bon; P B Sigler

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PubMed · 4019477

Crystals of crotoxin suitable for high resolution x-ray diffraction analysis.

A Achari·F R Radvanyi·D Scott·C Bon·P B Sigler

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Abstract

The phospholipasic presynaptic neurotoxin, crotoxin, has been crystallized in a morphology suitable for single crystal x-ray diffraction analysis. The conditions for growth and the unit cell parameters (P4(1)22 or P4(3)22, a = b = 38.5 A, c = 256.9 A, 1 molecule/asymmetric unit) are similar to the very thin plate-like crystals which have been studied with electron diffraction and electron microscopy by Chiu and his colleagues (Jeng, T.-W., Chiu, W., Zemlin, F., and Zeitler, E. (1984) J. Mol. Biol. 175, 93 - 97). These two macroscopic crystal morphologies of what is likely to be a very similar, if not identical, lattice structure will permit the complementary application of electron diffraction/microscopy and x-ray diffraction to understanding the structural basis of the interactions between a phospholipasic neurotoxin and its membrane target.

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Animals Crotalid Venoms Crotoxin Crystallization Microscopy, Electron Protein Conformation X-Ray Diffraction Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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A Achari, F R Radvanyi, D Scott, C Bon, P B Sigler. 1985-08-05. Crystals of crotoxin suitable for high resolution x-ray diffraction analysis.. https://pubmed.ncbi.nlm.nih.gov/4019477/

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